Coding
Part:BBa_K5403997:Design
Designed by: Lizette van der Ziel Group: iGEM24_TU-Eindhoven (2024-10-01)
Design considerations
The sequence is based on the wildtype GFP as given in the Protein Data Bank code '1W7S' (PDB ‘1W7S’, 2005), but missing the start codon, because another protein is to be fused to the N-terminus, see parts BBa_K5403014 - BBa_K5403020. The DNA-sequence was codon-optimized for expression in M. smegmatis.
Source
Aequoria victoria
References
RCSB PDB (2005). PDB - 1W7S: Wild-Type Aequorea victoria Green Fluorescent Protein. https://www.rcsb.org/structure/1W7S